Generator
BOG

Part:BBa_J45250:Experience

Designed by: André Green II   Group: iGEM06_MIT   (2006-10-28)

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Applications of BBa_J45250

Stationary phase banana odor production from the exogenous precursor isoamyl alcohol.

User Reviews

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Reshma Shetty

The stationary phase banana odor generator (BBa_J45250) produces a banana odor in culture when supplemented with isoamyl alcohol. As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density.

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Characterization

Transcriptional control of banana odor enzyme generator

Growth dependent banana odor production
To demonstrate growth phase dependent banana odor production, we compared the behavior of constitutive and stationary phase dependent banana odor generators (A and B, respectively). We measured isoamyl acetate concentrations of cultures of the constitutive and stationary phase banana odor generators at different culture cell densities (OD600nm) (C). As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density. By comparison, the constitutive banana odor generator produced more isoamyl acetate at lower cell densities than the stationary phase banana odor generator. To visually aid comparison of the two odor generators, an empirical fit to the data for each device is shown.